| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 100 µg |
goat anti-ECS (DYDDDDK) tag polyclonal antibody 8522
$426.00
Antibody summary
- Goat polyclonal to DYDDDDK tag
- Suitable for: ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg
goat anti-ECS (DYDDDDK) tag polyclonal antibody 8522
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500. Immunoprecipitation: use 1-4ug/mg lysate These are |
| Immunogen DDDDK conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Enteropeptidase (EC 3.4.21.9) (Enterokinase) (Serine protease 7) (Transmembrane protease serine 15) [Cleaved into: Enteropeptidase non-catalytic heavy chain; Enteropeptidase catalytic light chain] |
| Gene names TMPRSS15,TMPRSS15 ENTK PRSS7 |
| Protein family Peptidase S1 family |
| Mass 112935Da |
| Function Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.; EC=3.4.21.9; |
| Subellular location Membrane ; Single-pass type II membrane protein . |
| Tissues Intestinal brush border. |
| Structure Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond. |
| Post-translational modification The chains are derived from a single precursor that is cleaved by a trypsin-like protease. |
| Domain TOPO_DOM 2 |
| Involvement in disease DISEASE: Enterokinase deficiency (ENTKD) [MIM:226200]: Life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: P98073 |
| The UniProt Consortium |
Data
 |
| Detection of ECS (DYKDDDDK)-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified goat anti-ECS (DYKDDDDK) antibody 8522 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
|
| Detection of ECS (DYKDDDDK)-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified goat anti-ECS (DYKDDDDK) antibody 8522 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
|---|
Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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