| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | BRD2 |
| available sizes | 100 µg |
goat anti-BRD2 polyclonal antibody 8339
$518.00
Antibody summary
- Goat polyclonal to BRD2
- Suitable for: WB,IP
- Isotype: Whole IgG
- 100 µg
goat anti-BRD2 polyclonal antibody 8339
| antibody |
|---|
| Tested applications WB,IP |
| Recommended dilutions Immunoblotting: 0.025 - 0.1 ug/mL.Detection of human BRD2 in RIPA extract (10ug) of HeLa cells untreated (-) or treated (+) with calf intestinal phosphatase with #13020 at 0.05ug/mL. |
| Immunogen Synthetic peptide representing a portion of the protein between the putative nuclear localization signal and the ET domain |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This product is stable at 2 -8°C for 1 year. |
| Storage buffer Tris-citrate/phosphate buffer, pH 7 to 8 contai |
| Purity immunogen affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Bromodomain-containing protein 2 (O27.1.1) (Really interesting new gene 3 protein) |
| Gene names BRD2,BRD2 KIAA9001 RING3 |
| Mass 88061Da |
| Function FUNCTION: May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly. {ECO:0000250, ECO:0000269|PubMed:18406326}. |
| Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18406326, ECO:0000269|PubMed:25593309}. Note=Detected on chromatin and nucleosomes. {ECO:0000269|PubMed:18406326}. |
| Structure SUBUNIT: Homodimer. Interacts with E2F1 and with histone H4 acetylated at 'Lys-13'. {ECO:0000269|PubMed:17148447, ECO:0000269|PubMed:18406326}.; SUBUNIT: (Microbial infection) Interacts with herpes virus 8 protein LANA1. {ECO:0000269|PubMed:16227282}. |
| Target Relevance information above includes information from UniProt accession : P25440 |
| The UniProt Consortium |
Data
 |
| Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 µg ) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-BRD2 antibody 8339 (lot 8339-2) used for WB at 0.1 µg /ml. Detection: Chemiluminescence with an exposure time of 3 minutes. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot |
Documents
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