| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | AU5 |
| available sizes | 100 µg |
goat anti-AU5 polyclonal antibody 6772
$418.00
Antibody summary
- Goat polyclonal to AU5
- Suitable for: WB,IP,ICC,ELISA
- Isotype: Whole IgG
- 100 µg
goat anti-AU5 polyclonal antibody 6772
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:20,000 Immunocytochemistry: use at a dilution of 1:100-1:400 ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. Immunoprecipitation: use at 1-4ug/mg lysate. These ar |
| Immunogen AU5 (TDFYLK) conjugated to KLH. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS (PBS), pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| AU5 |
| Protein names AU5 epitope tag |
| Alternative names AU5 tag, AU5 epitope, TDFYLK tag |
| Function The AU5 tag is an artificial peptide epitope used for the detection and characterization of recombinant proteins using anti-AU5 antibodies. |
| Description The AU5 tag is a short peptide epitope derived from the VP1 capsid protein of bovine papillomavirus type 1 (BPV-1). It is used as a recombinant protein tag for detection, localization, and characterization of fusion proteins. |
| Keywords Epitope tag, Protein detection, Protein localization, Western blot, Recombinant protein |
| Sequence TDFYLK |
| Structure The AU5 tag is a 6-amino acid peptide epitope with the sequence TDFYLK derived from the VP1 protein of bovine papillomavirus type 1. |
| Function The AU5 tag is used as a molecular epitope tag for detection, localization, and characterization of recombinant proteins. |
| Detection AU5-tagged proteins can be detected using anti-AU5 antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, and immunoprecipitation assays. |
| Applications The AU5 tag is typically fused to recombinant proteins at the N- or C-terminus to facilitate detection while minimizing effects on protein structure and function. |
| Research relevance The AU5 tag is less commonly used than FLAG, HA, Myc, or V5 tags but remains useful in recombinant protein studies and multi-tag expression systems. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What are the recommended dilutions for using the goat anti-AU5 polyclonal antibody in different applications?
For immunoblotting, use a dilution of 1:1,000 to 1:20,000; for immunocytochemistry, use 1:100 to 1:400; for ELISA detection, use 1:1,000 to 1:30,000, and for ELISA coating, use 1:100 to 1:500. For immunoprecipitation, use 1 to 4 µg per mg of lysate.
How should the goat anti-AU5 polyclonal antibody be stored to maintain its stability?
This antibody is stable for at least one year when stored at 2-8°C in its supplied buffer.
What is the immunogen used to generate the goat anti-AU5 polyclonal antibody?
The immunogen is the AU5 peptide sequence TDFYLK conjugated to KLH.
Which secondary antibodies are compatible with the goat anti-AU5 polyclonal antibody?
Compatible secondaries include donkey anti-goat IgG, H&L chain specific antibodies conjugated to peroxidase, biotin, or FITC, some of which are crossabsorbed for enhanced specificity.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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