| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | AU1 |
| available sizes | 100 µg |
goat anti-AU1 polyclonal antibody 6665
$426.00
Antibody summary
- Goat polyclonal to AU1
- Suitable for: WB,IP,ICC,ELISA
- Isotype: Whole IgG
- 100 µg
goat anti-AU1 polyclonal antibody 6665
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:20,000 ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating use at 1:100-1:500 Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use at 1-4ug/mg lysate. These are recommended |
| Immunogen AU1 (DTYRYI) conjugated to KLH. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS (PBS), pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| AU1 |
| Protein names AU1 epitope tag |
| Alternative names AU1 tag, AU1 epitope, DTYRYI tag |
| Function The AU1 tag is an artificial peptide epitope used for detection, localization, and characterization of recombinant proteins using anti-AU1 antibodies. |
| Description The AU1 tag is a short peptide epitope derived from the VP1 structural protein of bovine papillomavirus type 1 (BPV-1). It is used as a recombinant protein tag for detection and characterization of fusion proteins. |
| Keywords Epitope tag, Protein detection, Recombinant protein, Immunofluorescence, Western blot |
| Sequence DTYRYI |
| Structure The AU1 tag is a 6-amino acid peptide epitope with the sequence DTYRYI derived from the VP1 protein of bovine papillomavirus type 1. |
| Function The AU1 tag is used as a molecular epitope tag for detection and characterization of recombinant proteins. |
| Detection AU1-tagged proteins can be detected using anti-AU1 antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, and immunoprecipitation assays. |
| Applications The AU1 tag is typically fused to recombinant proteins at the N- or C-terminus to facilitate detection without substantially affecting protein function. |
| Research relevance Although less common than FLAG, HA, Myc, or V5 tags, the AU1 tag remains useful in multi-tag experimental systems and recombinant protein studies. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What are the recommended applications and dilution ranges for the goat anti-AU1 polyclonal antibody (SKU 6665)?
The goat anti-AU1 polyclonal antibody is suitable for Western Blotting (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme-Linked Immunosorbent Assay (ELISA), and Immunoprecipitation (IP). Recommended dilutions are as follows: for immunoblotting, use 1:1,000 to 1:20,000; for ELISA detection, 1:1,000 to 1:30,000, and for coating, 1:100 to 1:500; for immunocytochemistry, 1:100 to 1:400; for immunoprecipitation, use 1-4 µg per mg of lysate.
How should the goat anti-AU1 polyclonal antibody be stored to maintain stability?
This antibody should be stored at 2-8°C, where it remains stable for at least one year. It is supplied in a liquid form with a storage buffer of PBS at pH 7.2 containing 0.09% sodium azide (NaN3) as a preservative.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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