| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Agarose immobilized, V5 |
| available sizes | 100 µg |
goat anti-Agarose Immobilized V5 polyclonal antibody 5981
$496.00
Antibody summary
- Goat polyclonal to Agarose Immobilized
- Suitable for: IP
- Isotype: Whole IgG
- 100 µg
goat anti-Agarose Immobilized V5 polyclonal antibody 5981
| antibody |
|---|
| Tested applications IP |
| Recommended dilutions Immunoprecipitation: use 15-25ul of gel slurry per 0.1-1mg of protein extract or lysate. These are recommended concentrations. End users should determine optimal concentrations for their applications. |
| Immunogen Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.09 |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| Biotechnology The V5 tag has become a valuable tool in scientific research, particularly in studies involving the expression and detection of recombinant proteins. The V5 tag is a short peptide sequence (GKPIPNPLLGLDST) that can be fused to the N or C terminus of a target protein, allowing for easy detection and purification. Researchers commonly utilize the V5 tag as an epitope for specific V5 tag antibodies, enabling convenient and sensitive visualization of the tagged protein in various experimental assays, including Western blotting and immunofluorescence. The small size of the V5 tag minimizes potential interference with the function and properties of the tagged protein, making it an attractive choice as a molecular tag. Additionally, the V5 tag can be used for protein purification using affinity chromatography, facilitating the isolation and characterization of the tagged protein. The widespread use of the V5 tag in research owes to its efficiency, reliability, and compatibility with various expression systems, making it an essential tool for investigating protein dynamics, interactions, and cellular localization in diverse biological contexts. |
Data
| No results found |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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