| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Agarose immobilized, V5 |
| available sizes | 100 µg |
goat anti-Agarose Immobilized V5 polyclonal antibody 5981
$496.00
Antibody summary
- Goat polyclonal to Agarose Immobilized
- Suitable for: IP
- Isotype: Whole IgG
- 100 µg
goat anti-Agarose Immobilized V5 polyclonal antibody 5981
| antibody |
|---|
| Tested applications IP |
| Recommended dilutions Immunoprecipitation: use 15-25ul of gel slurry per 0.1-1mg of protein extract or lysate. These are recommended concentrations. End users should determine optimal concentrations for their applications. |
| Immunogen Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.09 |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| V5 tag |
| Protein names V5 epitope tag |
| Alternative names V5 tag, V5 epitope, SV5 tag, Pk tag |
| Function The V5 tag is an artificial peptide epitope used for the detection, purification, and characterization of recombinant proteins. It is recognized by anti-V5 antibodies in applications including western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation. |
| Description The V5 tag is a synthetic peptide epitope derived from the P and V proteins of Human orthorubulavirus 5 (formerly Simian Virus 5, SV5). It is commonly fused to recombinant proteins for detection, purification, and localization studies. |
| Keywords Epitope tag, Protein detection, Protein purification, Recombinant protein |
| Sequence GKPIPNPLLGLDST |
| Structure The V5 tag is a 14-amino acid peptide epitope with the sequence GKPIPNPLLGLDST derived from the P and V proteins of Human orthorubulavirus 5 (formerly SV5). |
| Function The V5 tag is used as a molecular tag for the detection, purification, and localization of recombinant proteins. |
| Applications V5-tagged proteins can be detected by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation. |
| Research relevance The V5 tag is widely used in recombinant protein expression systems and serves as an alternative to FLAG, HA, Myc, and His tags. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications has the Goat anti-Agarose Immobilized V5 polyclonal antibody 5981 been tested for?
This antibody has been tested and is suitable for immunoprecipitation (IP) applications. It is also recommended for use in ICC/IF and Western blot (WB) assays.
How should I store the Goat anti-Agarose Immobilized V5 polyclonal antibody to maintain its stability?
The antibody should be stored in liquid form at 2-8°C where it remains stable for at least one year. It is supplied in phosphate buffered saline (PBS) containing 0.09% sodium azide.
What is the host species and clonality of this anti-Agarose Immobilized V5 antibody?
The antibody is a goat polyclonal antibody of the IgG isotype.
What is the recommended usage concentration for immunoprecipitation with this antibody?
For immunoprecipitation, it is recommended to use 15-25 µL of the gel slurry per 0.1-1 mg of protein extract or lysate, though end users should optimize concentrations for their specific applications.
What is the immunogen used to generate the Goat anti-Agarose Immobilized V5 polyclonal antibody 5981?
The immunogen is a synthetic peptide corresponding to amino acids 95-108 (GKPIPNPLLGLDST) of the RNA polymerase alpha subunit of simian virus 5, conjugated to keyhole limpet hemocyanin (KLH).
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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