| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 100 µg |
goat anti-6 His polyclonal antibody 9027
$409.00
Antibody summary
- Goat polyclonal to 6 His
- Suitable for: WB, ICC/IG, IP, ELISA
- Isotype: IgG
- 100 µg
goat anti-6 His polyclonal antibody 9027
| antibody |
|---|
| Database link: 6x His tag sequence HHHHHH |
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test. |
| Immunogen Highly purified HHHHHH conjugated with KLH (6xHIS) |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions -20°C for 2 years or more. °Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. |
| Storage buffer PBS, pH 7.2, 0.05% NaN3 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries Donkey anti goat |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| 6X His tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify 6X His tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, 6X His tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and 6x His tag |
| Biotechnology The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting. The versatility and simplicity of the 6x His tag have significantly advanced research efforts in protein expression, characterization, and functional analysis, allowing researchers to explore the structure and function of a wide range of proteins in diverse biological systems. |
Data
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Publications
Publications
| pmid | title | authors | citation |
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| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
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| Western blot ICC |
Documents
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