goat anti-6 His HRP polyclonal antibody 1003

$522.00

Antibody summary

Goat polyclonal to 6 His , HRP conjugate
Suitable for: WB,IHC,ICC,ELISA
Isotype: Whole IgG
100 µg

SKU: 1003parent Category: antibody Tags: 6X His tag, goat, polyclonal

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	goat
isotype	Whole IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	6 His
available sizes	100 µg

goat anti-6 His HRP polyclonal antibody 1003

antibody

Tested applications WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:5,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000-1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications.
Immunogen 6-His (HHHHHH) conjugated to KLH.
Size and concentration 100µg and 1 mg/mL
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at 2-8°C.
Storage buffer Phosphate Buffered Saline (PBS) containing 0.2%
Purity peptide affinty purifcation
Clonality polyclonal
Isotype IgG
Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control Goat polyclonal - Isotype Control

target relevance

Biotechnology
The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting. The versatility and simplicity of the 6x His tag have significantly advanced research efforts in protein expression, characterization, and functional analysis, allowing researchers to explore the structure and function of a wide range of proteins in diverse biological systems.

Data

benchmark-antibodies_anti-6_his_antibody_hrp_conjugate_1003_1.jpg

Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-6-His antibody 1003 used for WB at 0.2 µg /ml (1:5,000). Detection: Chemiluminescence with an exposure time of 3 minutes.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.