Cynomolgus/Rhesus macaque CD47 Protein 3223

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln19-Glu141

available size

Clear

SKU: 3223parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	F7A802
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
molecular weight	The protein has a predicted MW of 15 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus/Rhesus macaque CD47 Protein 3223

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Protein names Leukocyte surface antigen CD47 (Integrin-associated protein)
Gene names CD47,CD47
Mass 9544Da
Subellular location Cell membrane ; Multi-pass membrane protein. Membrane ; Multi-pass membrane protein .
Target Relevance information above includes information from UniProt accession: F7A802
The UniProt Consortium

Data

SPR with Cynomolgus/Rhesus macaque CD47 Protein

Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus/Rhesus macaque CD47 Protein

Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test).

HPLC of Cynomolgus/Rhesus macaque CD47 Protein

The purity of Cynomolgus/Rhesus macaque CD47 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus/Rhesus macaque CD47 Protein

Cynomolgus/Rhesus macaque CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.