SPR with Cynomolgus PD-1/PDCD1 Protein 4457

ELISA with Cynomolgus PD-1/PDCD1 Protein 4457

Cynomolgus PD-1/PDCD1 Protein 4457

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Pro21-Gln167

SKU: 4457parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	B0LAJ3
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Programmed cell death protein 1, also known as PD-1 and CD279, is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
molecular weight	The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus PD-1/PDCD1 Protein 4457

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Protein names Programmed cell death 1
Mass 9541Da
Target Relevance information above includes information from UniProt accession: B0LAJ3
The UniProt Consortium

Data

Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus PD-L1, His Tag with an affinity constant of 1.28 µM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus PD-1/PDCD1 Protein

Immobilized Cynomolgus PD-1, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 29.5ng/ml determined by ELISA (QC Test).

The purity of Cynomolgus PD-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus PD-1/PDCD1 Protein

Cynomolgus PD-1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.