Cynomolgus IL-1 Beta/IL-1F2 Protein 2779

$315.00 – $1,050.00

Summary

Expression: E.coli
Binding assay: Yes (SPR)
Amino Acid Range: Ala117-Ser268

available size

Clear

SKU: 2779parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A2K5VDC7
express system	E.coli
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
molecular weight	The protein has a predicted MW of 18.33 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus IL-1 Beta/IL-1F2 Protein 2779

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
Protein names Multifunctional fusion protein [Includes: Interleukin-1; Interleukin-1 beta]
Gene names IL1B,IL1B
Protein family IL-1 family
Mass 9541Da
Function Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Subellular location Cytoplasm, cytosol. Secreted. Lysosome. Secreted, extracellular exosome. Note=The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10.
Structure Monomer. Interacts with MEFV.
Target Relevance information above includes information from UniProt accession: A0A2K5VDC7
The UniProt Consortium

Data

SPR with Cynomolgus IL-1 Beta/IL-1F2 Protein

Human IL-1 RII, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-1 Beta, His Tag with an affinity constant of 1.40 nM as determined in SPR assay (Biacore T200).

HPLC of Cynomolgus IL-1 Beta/IL-1F2 Protein

The purity of Cynomolgus IL-1 Beta is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus IL-1 Beta/IL-1F2 Protein

Cynomolgus IL-1 Beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.