Cynomolgus CTLA-4/CD152 Protein 4271

$113.00 – $375.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala37-Ser160

SKU: 4271parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	G7PL88
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
molecular weight	The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus CTLA-4/CD152 Protein 4271

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
Protein names Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4)
Gene names CTLA4,CTLA4
Mass 24671Da
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane ; Single-pass type I membrane protein .
Target Relevance information above includes information from UniProt accession: G7PL88
The UniProt Consortium

Data

SPR with Cynomolgus CTLA-4/CD152 Protein

Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus CTLA-4/CD152 Protein

Immobilized Cynomolgus CTLA-4, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).

Immobilized Cynomolgus CTLA-4, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.

The purity of Cynomolgus CTLA-4 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus CTLA-4/CD152 Protein

Cynomolgus CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.