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Cynomolgus CD73/NT5E Protein 4245 – benchmark antibodies Cynomolgus CD73/NT5E Protein 4245
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ELISA with Cynomolgus CD73/NT5E Protein 4245
ELISA with Cynomolgus CD73/NT5E Protein 4245

Cynomolgus CD73/NT5E Protein 4245

$315.00 – $1,050.00

Summary

  • Expression: HEK293
  • Active: Yes (catalytic)
  • Amino Acid Range: Trp27-Cys554
SKU: 4245parent Categories: , Tag:
Weight 1 lbs
Dimensions 9 × 5 × 2 in
accession

EHH53214
express system

HEK293
product tag

C-His
purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background

CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.
molecular weight

The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
available size

100 µg, 500 µg
endotoxin

Less than 1EU per μg by the LAL method.

Cynomolgus CD73/NT5E Protein 4245
protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A�  deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Protein names
5'-nucleotidase (5'-NT) (EC 3.1.3.35) (EC 3.1.3.5) (EC 3.1.3.89) (EC 3.1.3.91) (EC 3.1.3.99) (5'-deoxynucleotidase) (Ecto-5'-nucleotidase) (IMP-specific 5'-nucleotidase) (Thymidylate 5'-phosphatase) (CD antigen CD73)
Gene names
NT5E,NT5E NT5 NTE
Protein family
5'-nucleotidase family
Mass
63368Da
Function
Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347).
Catalytic activity
CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5; Evidence=; CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate; Xref=Rhea:RHEA:36167, ChEBI:CHEBI:15377, ChEBI:CHEBI:18274, ChEBI:CHEBI:43474, ChEBI:CHEBI:65317; EC=3.1.3.89; Evidence=; CATALYTIC ACTIVITY: Reaction=dTMP + H2O = phosphate + thymidine; Xref=Rhea:RHEA:11080, ChEBI:CHEBI:15377, ChEBI:CHEBI:17748, ChEBI:CHEBI:43474, ChEBI:CHEBI:63528; EC=3.1.3.35; Evidence=; CATALYTIC ACTIVITY: Reaction=CMP + H2O = cytidine + phosphate; Xref=Rhea:RHEA:29367, ChEBI:CHEBI:15377, ChEBI:CHEBI:17562, ChEBI:CHEBI:43474, ChEBI:CHEBI:60377; EC=3.1.3.91; Evidence=; CATALYTIC ACTIVITY: Reaction=H2O + IMP = inosine + phosphate; Xref=Rhea:RHEA:27718, ChEBI:CHEBI:15377, ChEBI:CHEBI:17596, ChEBI:CHEBI:43474, ChEBI:CHEBI:58053; EC=3.1.3.99; Evidence=; CATALYTIC ACTIVITY: Reaction=AMP + H2O = adenosine + phosphate; Xref=Rhea:RHEA:29375, ChEBI:CHEBI:15377, ChEBI:CHEBI:16335, ChEBI:CHEBI:43474, ChEBI:CHEBI:456215; Evidence=; CATALYTIC ACTIVITY: Reaction=GMP + H2O = guanosine + phosphate; Xref=Rhea:RHEA:27714, ChEBI:CHEBI:15377, ChEBI:CHEBI:16750, ChEBI:CHEBI:43474, ChEBI:CHEBI:58115; Evidence=; CATALYTIC ACTIVITY: Reaction=H2O + UMP = phosphate + uridine; Xref=Rhea:RHEA:29359, ChEBI:CHEBI:15377, ChEBI:CHEBI:16704, ChEBI:CHEBI:43474, ChEBI:CHEBI:57865; Evidence=; CATALYTIC ACTIVITY: Reaction=dAMP + H2O = 2'-deoxyadenosine + phosphate; Xref=Rhea:RHEA:29371, ChEBI:CHEBI:15377, ChEBI:CHEBI:17256, ChEBI:CHEBI:43474, ChEBI:CHEBI:58245; Evidence=; CATALYTIC ACTIVITY: Reaction=dCMP + H2O = 2'-deoxycytidine + phosphate; Xref=Rhea:RHEA:29363, ChEBI:CHEBI:15377, ChEBI:CHEBI:15698, ChEBI:CHEBI:43474, ChEBI:CHEBI:57566; Evidence=;
Subellular location
Cell membrane ; Lipid-anchor, GPI-anchor .
Structure
Homodimer.
Target Relevance information above includes information from UniProt accession: P21589
The UniProt Consortium

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