Cynomolgus CD200 R1/CRTR2 Protein 3203

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu25-Leu267

available size

Clear

SKU: 3203parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_005548207
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
molecular weight	The protein has a predicted MW of 28.09 kDa. Due to glycosylation, the protein migrates to 67-77 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus CD200 R1/CRTR2 Protein 3203

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
Protein names Cell surface glycoprotein CD200 receptor 1 (CD200 cell surface glycoprotein receptor) (Cell surface glycoprotein OX2 receptor 1)
Protein family CD200R family
Mass 39041Da
Function Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. {ECO:0000269\|PubMed:12960329}.
Subellular location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.
Tissues Expressed in granulocytes, monocytes, most T-cells, neutrophils, basophils and a subset of NK, NKT and B-cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells. {ECO:0000269\|PubMed:12960329}.
Structure CD200 and CD200R1 interact via their respective N-terminal Ig-like domains (By similarity). Interacts with Human herpesvirus 8 vOX2 protein. {ECO:0000250}.; (Microbial infection) Interacts with human herpesvirus 8/HHV-8 protein vOX2/K14. {ECO:0000269\|Ref.2}.
Target Relevance information above includes information from UniProt accession: Q8TD46
The UniProt Consortium

Data

SPR with Cynomolgus CD200 R1/CRTR2 Protein

Cynomolgus CD200 R1, His Tag immobilized on CM5 Chip can bind Cynomolgus CD200, His Tag with an affinity constant of 35.84 nM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus CD200 R1/CRTR2 Protein

Immobilized Cynomolgus CD200 R1, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).

HPLC of Cynomolgus CD200 R1/CRTR2 Protein

The purity of Cynomolgus CD200 R1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus CD200 R1/CRTR2 Protein

Cynomolgus CD200 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.