SPR with Cynomolgus B7-H2/ICOSLG Protein 4256

ELISA with Cynomolgus B7-H2/ICOSLG Protein 4256

Cynomolgus B7-H2/ICOSLG Protein 4256

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asp19-Thr256

SKU: 4256parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_005548617
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
molecular weight	The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus B7-H2/ICOSLG Protein 4256

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Protein names ICOS ligand (B7 homolog 2) (B7-H2) (B7-like protein Gl50) (B7-related protein 1) (B7RP-1) (CD antigen CD275)
Gene names ICOSLG,ICOSLG B7H2 B7RP1 ICOSL KIAA0653
Protein family Immunoglobulin superfamily, BTN/MOG family
Mass 33349Da
Function FUNCTION: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed:11007762, PubMed:11023515, PubMed:30498080). Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP) (PubMed:30498080). {ECO:0000250, ECO:0000269\|PubMed:11007762, ECO:0000269\|PubMed:11023515, ECO:0000269\|PubMed:30498080}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:11007762, ECO:0000269\|PubMed:30498080}; Single-pass type I membrane protein {ECO:0000255}.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane {ECO:0000269\|PubMed:11390480}.
Tissues TISSUE SPECIFICITY: Expressed on peripheral blood B-cells and monocytes, as well as on monocyte-derived dendritic cells (at protein level). {ECO:0000269\|PubMed:10779774, ECO:0000269\|PubMed:11007762, ECO:0000269\|PubMed:11023515}.; TISSUE SPECIFICITY: [Isoform 1]: Widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil). {ECO:0000269\|PubMed:11390480}.; TISSUE SPECIFICITY: [Isoform 2]: Detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. {ECO:0000269\|PubMed:11390480}.
Structure SUBUNIT: Interacts with CTLA4 (in vitro). {ECO:0000269\|PubMed:28484017}.
Target Relevance information above includes information from UniProt accession: O75144
The UniProt Consortium

Data

SPR with Cynomolgus B7-H2/ICOSLG Protein

Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus B7-H2/ICOSLG Protein

Immobilized Human ICOS, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Cynomolgus B7-H2, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test).

The purity of Cynomolgus B7-H2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus B7-H2/ICOSLG Protein

Cynomolgus B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.