Cynomolgus B7-2/CD86 Protein 4230

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Tyr26-His240

SKU: 4230parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	G7NXR4
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
molecular weight	The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus B7-2/CD86 Protein 4230

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Protein names Ig-like domain-containing protein
Mass 9541Da
Subellular location Membrane ; Single-pass type I membrane protein .
Target Relevance information above includes information from UniProt accession: G7NXR4
The UniProt Consortium

Data

Cynomolgus B7-2, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 0.102 µM as determined in SPR assay (Biacore T200).

Immobilized Cynomolgus B7-2, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).

The purity of Cynomolgus B7-2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus B7-2/CD86 Protein

Cynomolgus B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.