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Bordetella pertussis IgA ELISA kit-SERION <i>classic</i> ESR120A - Image 2

Bordetella pertussis IgA ELISA kit-SERION classic ESR120A

$364.00

Summary

Virion/Serion Diagnostic Kit for research use (RUO)
Bordetella pertussis IgA ELISA Kit
Suitable for IgA detection
Ready-to-use
96 tests

SKU: ESR120A Category: Virion/Serion kits Tags: Bordetella pertussis, ELISA, IgA

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Bordetella pertussis reactive IgA
species reactivity	Bordetella pertussis (whooping cough)
applications	ELISA
assay type	Indirect & quantitative
available sizes	96 tests

Bordetella pertussis IgA ELISA Kit ESR120A

Assay type
Indirect ELISA

Research area
Infectious Disease

Sample type
Serum, plasma, whole blood

Components

Break apart microtiter test strips each with antigen coated single wells	8 x 12 (96 Total)
Standard serum (ready-to-use)	2 x 2 mL
Negative control serum (ready-to-use)	2 mL
Anti-human-IgA-conjugate (ready-to-use)	13 mL
Washing solution concentrate (sufficient for 1000ml)	33.3 mL
Dilution buffer	2 x 50 mL
Stopping solution	15 mL
Substrate (ready-to-use)	13 mL
Quality control certificate with standard curve and evaluation table	1

Storage
Store at 2-8°C.

target relevance

human antibody anti-Bordetella pertussis

Bordetella pertussis

Description
Bacterium that causes whooping cough (pertussis)

Structure
Bordetella pertussis is a small, Gram-negative, aerobic coccobacillus belonging to the family Alcaligenaceae. The bacterium is a strictly human pathogen and the primary causative agent of pertussis, commonly known as whooping cough. B. pertussis colonizes the ciliated epithelium of the upper respiratory tract through the action of multiple adhesins, including filamentous hemagglutinin, pertactin, and fimbriae. The organism produces several virulence factors, most notably pertussis toxin, adenylate cyclase toxin, and tracheal cytotoxin, which interfere with host immune responses and contribute to respiratory tract damage. Despite widespread vaccination programs, Bordetella pertussis remains a significant cause of respiratory disease worldwide, particularly among infants and inadequately vaccinated populations.

Diagnosis
The diagnosis of Bordetella pertussis infection is performed using microbiological, molecular, and serological methods. Polymerase chain reaction (PCR) testing of nasopharyngeal specimens is the preferred laboratory method during the early stages of infection due to its high sensitivity and rapid turnaround time. Culture of B. pertussis from nasopharyngeal swabs remains the definitive diagnostic method but is less sensitive and requires specialized media and prolonged incubation. Serological assays, including ELISA-based tests that detect antibodies against pertussis toxin and other bacterial antigens, are useful in later stages of disease when bacterial shedding has decreased. Laboratory diagnosis is particularly important because clinical presentation may overlap with other respiratory infections.

Symptoms
Bordetella pertussis infection is highly contagious and spreads through respiratory droplets generated during coughing or sneezing. Following an incubation period of approximately seven to ten days, disease typically progresses through three stages. The catarrhal stage resembles a common upper respiratory infection with symptoms including runny nose, mild fever, sneezing, and occasional cough. This is followed by the paroxysmal stage, characterized by severe repetitive coughing fits that may be followed by the characteristic inspiratory 'whoop,' post-tussive vomiting, and exhaustion. In infants, apnea may occur and can be life-threatening. The final convalescent stage involves gradual recovery over weeks to months, although coughing episodes may persist. Complications include pneumonia, seizures, encephalopathy, rib fractures, and, particularly in young infants, death.

Data

FAQ & Publications

Frequently Asked Questions

What sample types are compatible with the Bordetella pertussis IgA ELISA Kit ESR120A?

The Bordetella pertussis IgA ELISA Kit ESR120A is compatible with serum, plasma, and whole blood samples for the detection of Bordetella pertussis reactive IgA antibodies.

How should the Bordetella pertussis IgA ELISA Kit ESR120A be stored to maintain its stability?

The kit should be stored at 2-8°C to ensure the stability and integrity of all its components until use.

What is the assay type and detection method used in the Bordetella pertussis IgA ELISA Kit ESR120A?

This kit employs an indirect and quantitative ELISA assay format designed for the detection of IgA antibodies reactive to Bordetella pertussis antigens.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
ESR120A protocol

Documents

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Bordetella pertussis IgA ELISA kit-SERION classic ESR120A

Data

FAQ & Publications

Protocols

Documents

Related Technical Resources & Posts

Reviews

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