ELISA with Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Mo 5002

HPLC of Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monom 5002

SDS-PAGE gel of Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQ 5002

Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein 5002

$310.00 – $1,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide

SKU: 5002parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
molecular weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein 5002

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Protein names MHC class I antigen
Gene names Mamu-AG,Mamu-AG
Protein family MHC class I family
Mass 9544Da
Function Involved in the presentation of foreign antigens to the immune system.
Subellular location Membrane ; Single-pass type I membrane protein .
Target Relevance information above includes information from UniProt accession: O02948
The UniProt Consortium

Data

ELISA with Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Mo

Immobilized Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL), His Tag at 2µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 75.8ng/ml determined by ELISA.

HPLC of Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monom

The purity of Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQ

Biotinylated Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.