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Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) 3865

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Binding assay: Yes (SPR)
  • Amino Acid Range: Phe19-Thr238
SKU: 3865parent Categories: , Tag:
Weight 1 lbs
Dimensions 9 × 5 × 2 in
express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

molecular weight

The protein has a predicted MW of 25.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) 3865

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

SPR with Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling
Biotinylated Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-1 , hFc Tag with an affinity constant of 0.31 µM as determined in SPR assay (Biacore T200).
HPLC of Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling)
The purity of Biotinylated Mouse PD-L1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine L
Biotinylated Mouse PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product

Documents

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