SDS-PAGE gel of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer P 2076

Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein 2076

$310.00 – $1,250.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide

SKU: 2076parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
molecular weight	The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein 2076

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
Protein names H-2 class I histocompatibility antigen, K-B alpha chain (H-2K(B))
Gene names H2-K1,H2-K1 H2-K
Protein family MHC class I family
Mass 10090Da
Function Involved in the presentation of foreign antigens to the immune system.
Subellular location Membrane; Single-pass type I membrane protein.
Structure Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).
Target Relevance information above includes information from UniProt accession: P01901
The UniProt Consortium

Data

HPLC of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein

The purity of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer P

Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.