ELISA with Biotinylated Human P-Selectin/CD62P Protein 4676

Biotinylated Human P-Selectin/CD62P Protein 4676

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Trp42-Ala771

available size

Clear

SKU: 4676parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
molecular weight	The protein has a predicted MW of 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human P-Selectin/CD62P Protein 4676

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
Protein names P-selectin (CD62 antigen-like family member P) (Granule membrane protein 140) (GMP-140) (Leukocyte-endothelial cell adhesion molecule 3) (LECAM3) (Platelet activation dependent granule-external membrane protein) (PADGEM) (CD antigen CD62P)
Gene names SELP,SELP GMRP GRMP
Protein family Selectin/LECAM family
Mass 90820Da
Function FUNCTION: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B:ITGB3) and integrin alpha-V/beta-3 (ITGAV:ITGB3) (PubMed:37184585). {ECO:0000269\|PubMed:11081633, ECO:0000269\|PubMed:28011641, ECO:0000269\|PubMed:37184585, ECO:0000269\|PubMed:7585950}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:28011641}; Single-pass type I membrane protein {ECO:0000305\|PubMed:28011641}.
Tissues TISSUE SPECIFICITY: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Structure SUBUNIT: Interacts with SNX17. Interacts with SELPLG/PSGL1 and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of SELPLG and PODXL2, and specific tyrosine sulfation on SELPLG. Interacts (via C-type lectin domain) with alpha-IIb/beta3 integrin ITGA2B:ITGB3 and alpha-V/beta-3 integrin ITGAV:ITGB3 (PubMed:37184585). Interacts with alpha5/beta1 integrin ITGA5:ITGB1 and alpha4/beta1 integrin ITGA4:ITGB (PubMed:37184585). {ECO:0000269\|PubMed:11081633, ECO:0000269\|PubMed:11237770, ECO:0000269\|PubMed:15769472, ECO:0000269\|PubMed:18606703, ECO:0000269\|PubMed:37184585, ECO:0000269\|PubMed:7559387, ECO:0000269\|PubMed:7585949, ECO:0000269\|PubMed:7585950}.
Domain DOMAIN: The C-type lectin domain is required for binding to integrins (PubMed:37184585). Binding to soluble integrins alpha-V/beta-3 (ITGAV:ITGB3) and alpha-IIb/beta3 (ITGA2B:ITGB) is cation-dependent in order of preference of 1 mM Mn(2+) > Mg(2+) > Ca(2+) (PubMed:37184585). Binds to the allosteric site (site 2) of integrins and activates them (PubMed:37184585). The interaction with integrins may mediate cell-cell interactions and cell adhesion (PubMed:37184585). {ECO:0000269\|PubMed:37184585}.
Target Relevance information above includes information from UniProt accession: P16109
The UniProt Consortium

Data

ELISA with Biotinylated Human P-Selectin/CD62P Protein

Immobilized Biotinylated Human P-Selectin, His Tag at 1µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.

HPLC of Biotinylated Human P-Selectin/CD62P Protein

The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human P-Selectin/CD62P Protein

Biotinylated Human P-Selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.