Biotinylated Human Nectin-4 Protein IgV Domain 4810

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly32-Leu146

SKU: 4810parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
molecular weight	The protein has a predicted MW of 15.8 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human Nectin-4 Protein IgV Domain 4810

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Protein names Nectin-4 (Ig superfamily receptor LNIR) (Nectin cell adhesion molecule 4) (Poliovirus receptor-related protein 4) [Cleaved into: Processed poliovirus receptor-related protein 4]
Gene names NECTIN4,NECTIN4 LNIR PRR4 PVRL4
Protein family Nectin family
Mass 9606Da
Function Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.; (Microbial infection) Acts as a receptor for measles virus.
Subellular location Cell membrane ; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; [Processed poliovirus receptor-related protein 4]: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625).
Tissues Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
Structure Self-associates. Interacts via its Ig-like V-type domain with NECTIN1 Ig-like V-type domain. Interacts via its C-terminus with AFDN.; (Microbial infection) Interacts with measles virus Hemagglutinin protein (PubMed:22048310, PubMed:23202587).
Post-translational modification The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.
Target Relevance information above includes information from UniProt accession: Q96NY8
The UniProt Consortium

ELISA with Biotinylated Human Nectin-4 Protein IgV Domain

Immobilized Anti-Nectin-4 Antibody, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human Nectin-4 IgV Domain, His Tag with the EC50 of 30.9ng/ml determined by ELISA.

HPLC of Biotinylated Human Nectin-4 Protein IgV Domain

The purity of Biotinylated Human Nectin-4 IgV Domain is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human Nectin-4 Protein IgV Domain

Biotinylated Human Nectin-4 IgV Domain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.