Biotinylated Human IL-23 alpha&IL-12 beta Protein 3058

$675.00 – $2,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)

SKU: 3058parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.
molecular weight	The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation, the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human IL-23 alpha&IL-12 beta Protein 3058

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.
Protein names Interleukin-23 subunit alpha (IL-23 subunit alpha) (IL-23-A) (Interleukin-23 subunit p19) (IL-23p19)
Gene names IL23A,IL23A SGRF UNQ2498/PRO5798
Protein family IL-6 superfamily
Mass 9606Da
Function Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed:11114383). Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed:29287995, PubMed:32474165, PubMed:33606986). This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A (PubMed:12023369). In turn, participates in the early and effective intracellular bacterial clearance (PubMed:32474165). Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells (PubMed:17676044).
Subellular location Secreted. Note=Secreted upon association with IL12B.
Tissues Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
Structure Heterodimer with IL12B; disulfide-linked (PubMed:11114383, PubMed:18680750). The heterodimer is known as interleukin IL-23 (PubMed:11114383, PubMed:18680750). Interacts with IL23R; this interaction enables recruitment of IL12RB1 (PubMed:29287995, PubMed:33606986).
Target Relevance information above includes information from UniProt accession: Q9NPF7
The UniProt Consortium

ELISA with Biotinylated Human IL-23 alpha&IL-12 beta Protein

Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA (QC Test).

Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.

HPLC of Biotinylated Human IL-23 alpha&IL-12 beta Protein

The purity of Biotinylated Human IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human IL-23 alpha&IL-12 beta Protein

Biotinylated Human IL-23 alpha&IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.