Biotinylated Human FGL1 Protein (Primary Amine Labeling) 2666

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asp64-Asn305

available size

Clear

SKU: 2666parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	N-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
background	Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
molecular weight	The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human FGL1 Protein (Primary Amine Labeling) 2666

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Protein names Fibrinogen-like protein 1 (HP-041) (Hepassocin) (HPS) (Hepatocyte-derived fibrinogen-related protein 1) (HFREP-1) (Liver fibrinogen-related protein 1) (LFIRE-1)
Gene names FGL1,FGL1 HFREP1
Mass 9606Da
Function Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 (PubMed:30580966). Responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed:30580966). Secreted by, and promotes growth of, hepatocytes (PubMed:11470158, PubMed:19880967).
Subellular location Secreted. Note=Secreted in the blood plasma.
Tissues Under normal conditions, liver-specific.
Structure Homodimer (PubMed:11470158). Interacts (via the Fibrinogen C-terminal domain) with LAG3 (via Ig-like domains 1 and 2) (PubMed:30580966).
Target Relevance information above includes information from UniProt accession: Q08830
The UniProt Consortium

Data

ELISA with Biotinylated Human FGL1 Protein (Primary Amine Labeling)

Immobilized Human LAG3, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human FGL1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.

HPLC of Biotinylated Human FGL1 Protein (Primary Amine Labeling)

The purity of Biotinylated Human FGL1 is greater than 90% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human FGL1 Protein (Primary Amine Labeling

Biotinylated Human FGL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.