Biotinylated Human FGF21 Protein 2962

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: His29-Ser209

SKU: 2962parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	N-mFc-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
molecular weight	The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human FGF21 Protein 2962
protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
Protein names
Fibroblast growth factor 21 (FGF-21)
Gene names
FGF21,FGF21 UNQ3115/PRO10196
Protein family
Heparin-binding growth factors family
Mass
9606Da
Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
Subellular location
Secreted .
Structure
Interacts (via C-terminus) with KLB; this interaction is direct. Interacts with FGFR4.
Target Relevance information above includes information from UniProt accession: Q9NSA1
The UniProt Consortium

ELISA with Biotinylated Human FGF21 Protein

Immobilized Biotinylated Human FGF21, mFc Tag at 5µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.71µg/ml determined by ELISA.

HPLC of Biotinylated Human FGF21 Protein

The purity of Biotinylated Human FGF21 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human FGF21 Protein

Biotinylated Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.