Biotinylated Human CCR2b Protein-VLP 3851

$1,969.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Met1-Leu360

SKU: 3851parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P41597
express system	HEK293
product tag
purity	> 95% as determined by HPLC
background	The chemokine (C-C motif) receptor 2B (CCR2B) is one of the two isoforms of the receptor for monocyte chemoattractant protein-1 (CCL2), the major chemoattractant for monocytes, involved in an array of chronic inflammatory diseases. The actin-binding protein filamin A (FLNa) as a protein that associates with the carboxyl-terminal tail of CCR2B. FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures.
molecular weight	The target protein has a predicted MW of 42.9kDa.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μl by the LAL method.

Biotinylated Human CCR2b Protein-VLP 3851
protein
Size and concentration
100, 500µg and liquid
Form
Liquid
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped with dry ice.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The chemokine (C-C motif) receptor 2B (CCR2B) is one of the two isoforms of the receptor for monocyte chemoattractant protein-1 (CCL2), the major chemoattractant for monocytes, involved in an array of chronic inflammatory diseases. The actin-binding protein filamin A (FLNa) as a protein that associates with the carboxyl-terminal tail of CCR2B. FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures.
Protein names
C-C chemokine receptor type 2 (C-C CKR-2) (CC-CKR-2) (CCR-2) (CCR2) (Monocyte chemoattractant protein 1 receptor) (MCP-1-R) (CD antigen CD192)
Gene names
CCR2,CCR2 CMKBR2
Protein family
G-protein coupled receptor 1 family
Mass
9606Da
Function
Key functional receptor for CCL2 but can also bind CCL7 and CCL12 (PubMed:23408426, PubMed:8048929, PubMed:8146186). Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (Probable). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B (PubMed:23938203). Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (By similarity). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (By similarity).; (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
Subellular location
Cell membrane ; Multi-pass membrane protein. Note=The chemoattractant receptors are distributed throughout the cell surface; after stimulation with a ligand, such as CCL2, they are rapidly recruited into microdomain clusters at the cell membrane.
Tissues
Expressed by monocytes and IL2-activated NK cells.
Structure
Interacts with ARRB1 (PubMed:9501202). Interacts (via extracellular N-terminal region) with beta-defensin DEFB106A/DEFB106B; this interaction may preferentially require specific tyrosine sulfation on CCR2 (PubMed:23938203). Interacts with NUP85; the interaction is required for CCR2 clusters formation on the cell membrane and CCR2 signaling (PubMed:15995708, PubMed:25283965).; (Microbial infection) Binds to HIV-1 Tat.
Post-translational modification
N-glycosylated.; Sulfation increases the affinity for both monomeric and dimeric CCL2 with stronger binding to the monomeric form (PubMed:11046064, PubMed:23408426). Binding of sulfated CCR2 to CCL2 promotes conversion of CCL2 from dimer to monomer (PubMed:11046064, PubMed:23408426).
Target Relevance information above includes information from UniProt accession: P41597
The UniProt Consortium

SPR with Biotinylated Human CCR2b Protein-VLP

Biotinylated Human CCR2b VLP captured on SA Chip can bind Anti-CCR2b Antibody with an affinity constant of 5.24 nM as determined in SPR assay (Biacore T200).

HPLC of Biotinylated Human CCR2b Protein-VLP

The purity of Biotinylated Human CCR2b VLP is greater than 95% as determined by SEC-HPLC.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.