Biotinylated Human CCL5 (Rantes) Protein 3294

$525.00 – $1,750.00

Summary

Expression: E.coli
Functional: Yes (ELISA)
Amino Acid Range: Ser24-Ser91

SKU: 3294parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	E.coli
product tag	N-His-Avi
purity	> 95% as determined by Tris-Bis PAGE
background	The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
molecular weight	The protein has a predicted MW of 10.89 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human CCL5 (Rantes) Protein 3294
protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
Protein names
C-C motif chemokine 5 (EoCP) (Eosinophil chemotactic cytokine) (SIS-delta) (Small-inducible cytokine A5) (T cell-specific protein P228) (TCP228) (T-cell-specific protein RANTES) [Cleaved into: RANTES(3-68); RANTES(4-68)]
Gene names
CCL5,CCL5 D17S136E SCYA5
Protein family
Intercrine beta (chemokine CC) family
Mass
9990Da
Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) (PubMed:1380064, PubMed:15923218, PubMed:16791620, PubMed:8525373, PubMed:9516414). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).
Subellular location
Secreted.
Tissues
Expressed in the follicular fluid (at protein level). T-cell and macrophage specific.
Structure
Homo and heterooligomers with other chemokines. Interacts with the brown dog tick evasin-4.
Post-translational modification
N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.; N-terminal processed form RANTES(4-68) is produced by proteolytic cleavage by cathepsin CTSG.; The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
Target Relevance information above includes information from UniProt accession: P13501
The UniProt Consortium

ELISA with Biotinylated Human CCL5 Protein

Immobilized Biotinylated Human CCL5, His Tag at 1µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.

SDS-PAGE gel of Biotinylated Human CCL5 Protein

Biotinylated Human CCL5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.