Biotinylated Human B7-H5/Gi24/VISTA Protein 2137

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Phe33-Ala194

available size

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SKU: 2137parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	B7-H5, also known as VISTA, B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.
molecular weight	The protein has a predicted MW of 21.04 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human B7-H5/Gi24/VISTA Protein 2137

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.
Protein names V-type immunoglobulin domain-containing suppressor of T-cell activation (Platelet receptor Gi24) (Stress-induced secreted protein-1) (Sisp-1) (V-set domain-containing immunoregulatory receptor) (V-set immunoregulatory receptor)
Mass 33908Da
Function Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777). {ECO:0000250\|UniProtKB:Q9D659, ECO:0000269\|PubMed:20666777, ECO:0000269\|PubMed:24691993}.
Subellular location Cell membrane {ECO:0000269\|PubMed:20666777, ECO:0000269\|PubMed:24691993}; Single-pass type I membrane protein {ECO:0000255}.
Tissues Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta, spleen, plasma blood leukocytes, and lung. Expressed at moderate levels in lymph node, bone marrow, fat, uterus, and trachea. Has low expression levels in other tissues. {ECO:0000269\|PubMed:24691993}.
Post-translational modification At the cell surface, may be cleaved by MMP14. {ECO:0000269\|PubMed:20666777}.; N-glycosylated. {ECO:0000250\|UniProtKB:Q9D659}.
Target Relevance information above includes information from UniProt accession: Q9H7M9
The UniProt Consortium

Data

ELISA with Biotinylated Human B7-H5/Gi24/VISTA Protein

Immobilized Biotinylated Human B7-H5, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

HPLC of Biotinylated Human B7-H5/Gi24/VISTA Protein

The purity of Biotinylated Human B7-H5 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human B7-H5/Gi24/VISTA Protein

Biotinylated Human B7-H5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.