ELISA with Biotinylated Human B7-1/CD80 Protein 4321

Biotinylated Human B7-1/CD80 Protein 4321

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val35-Asn242

available size

Clear

SKU: 4321parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
molecular weight	The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human B7-1/CD80 Protein 4321

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Protein names T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (BB1) (CTLA-4 counter-receptor B7.1) (B7) (CD antigen CD80)
Gene names CD80,CD80 CD28LG CD28LG1 LAB7
Mass 33048Da
Function FUNCTION: Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed:38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed:10438913). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed:12121659). Also acts as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed:36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity). {ECO:0000250\|UniProtKB:Q00609, ECO:0000269\|PubMed:10438913, ECO:0000269\|PubMed:10583602, ECO:0000269\|PubMed:12121659, ECO:0000269\|PubMed:12196291, ECO:0000269\|PubMed:36727298, ECO:0000269\|PubMed:38467718}.; FUNCTION: (Microbial infection) Acts as a receptor for adenovirus subgroup B. {ECO:0000269\|PubMed:16920215}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:38467718}; Single-pass type I membrane protein.
Tissues TISSUE SPECIFICITY: Expressed on activated B-cells, macrophages and dendritic cells.
Structure SUBUNIT: Homodimer. Interacts with CTLA4; this interaction inhibits T-cell activation (PubMed:10583602, PubMed:11279502). Interacts with PDL1/CD274; this interaction blocks PDL1/PDCD1 binding and thus PDL1/CD274 inhibitory function (PubMed:36727298). Interacts with CD28 (PubMed:12196291). {ECO:0000269\|PubMed:10583602, ECO:0000269\|PubMed:11279502, ECO:0000269\|PubMed:12196291, ECO:0000269\|PubMed:36727298}.; SUBUNIT: (Microbial infection) Interacts with adenovirus subgroup B fiber proteins. {ECO:0000269\|PubMed:16920215}.; SUBUNIT: (Microbial infection) Interacts with Orthopoxvirus OPG038/M2 protein, inhibiting the interaction with CTLA4/CD152. {ECO:0000269\|PubMed:30918073}.
Post-translational modification PTM: Palmitoylated by ZDHHC20; palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization. {ECO:0000269\|PubMed:38467718}.
Target Relevance information above includes information from UniProt accession: P33681
The UniProt Consortium

Data

ELISA with Biotinylated Human B7-1/CD80 Protein

Immobilized Biotinylated Human B7-1, His Tag at 0.5µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.

HPLC of Biotinylated Human B7-1/CD80 Protein

The purity of Biotinylated Human B7-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human B7-1/CD80 Protein

Biotinylated Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.