ELISA with Biotinylated Human B7-1/CD80 Protein 4321

Biotinylated Human B7-1/CD80 Protein 4321

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val35-Asn242

SKU: 4321parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
molecular weight	The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human B7-1/CD80 Protein 4321

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Protein names T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (BB1) (CTLA-4 counter-receptor B7.1) (B7) (CD antigen CD80)
Gene names CD80,CD80 CD28LG CD28LG1 LAB7
Mass 9606Da
Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.; (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Expressed on activated B-cells, macrophages and dendritic cells.
Structure Homodimer; CD80 dimers on the antigen presenting cells (APCs) bridge CTLA4/CD152 dimers on T-cells in a periodic zipper-like arrangement.; (Microbial infection) Interacts with adenovirus subgroup B fiber proteins.; (Microbial infection) Interacts with Orthopoxvirus OPG038/M2 protein, inhibiting the interaction with CTLA4/CD152.
Target Relevance information above includes information from UniProt accession: P33681
The UniProt Consortium

Data

ELISA with Biotinylated Human B7-1/CD80 Protein

Immobilized Biotinylated Human B7-1, His Tag at 0.5µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.

HPLC of Biotinylated Human B7-1/CD80 Protein

The purity of Biotinylated Human B7-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human B7-1/CD80 Protein

Biotinylated Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.