ELISA with Biotinylated Human Activin RIIA/ACVR2A?Protein 2046

Biotinylated Human Activin RIIA/ACVR2A Protein 2046

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala20-Pro135

SKU: 2046parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-hFc-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer.
molecular weight	The protein has a predicted MW of 41.16 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human Activin RIIA/ACVR2A Protein 2046

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer.
Protein names Activin receptor type-2A (EC 2.7.11.30) (Activin receptor type IIA) (ACTR-IIA) (ACTRIIA)
Gene names ACVR2A,ACVR2A ACVR2
Protein family Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfam
Mass 9606Da
Function On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A (PubMed:17911401). Mediates induction of adipogenesis by GDF6 (By similarity).
Subellular location Cell membrane ; Single-pass type I membrane protein .
Structure Part of a complex consisting of MAGI2/ARIP1, ACVR2A, ACVR1B and SMAD3 (By similarity). Interacts with MAGI2/ARIP1 (By similarity). Interacts with type I receptor ACVR1 (PubMed:17911401). Interacts with BMP7 (PubMed:12667445). Interacts with TSC22D1/TSC-22 (PubMed:21791611).
Target Relevance information above includes information from UniProt accession: P27037
The UniProt Consortium

Data

ELISA with Biotinylated Human Activin RIIA/ACVR2A-Protein

Immobilized Human Activin A, No Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human Activin RIIA, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA.

HPLC of Biotinylated Human Activin RIIA/ACVR2A~-Protein

The purity of Biotinylated Human Activin RIIA is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human Activin RIIA/ACVR2A~-Protein

Biotinylated Human Activin RIIA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.