Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein 4197

$600.00 – $2,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asp22-Thr48

SKU: 4197parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-hFc-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
molecular weight	The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein 4197
protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

ELISA with Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein

Immobilized Anti-CD3 Antibody, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.

HPLC of Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein

The purity of Biotinylated Cynomolgus CD3E 1-27 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein

Biotinylated Cynomolgus CD3E 1-27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.