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beta Tubulin antibodies
Beta-tubulin, a key structural component of microtubules, plays a vital role in maintaining cellular architecture, supporting intracellular transport, and facilitating critical processes such as mitosis. Given its essential role in microtubule formation and consistent expression across various cell types, beta-tubulin is frequently used as a reliable loading control in Western blotting (WB) and other techniques like immunoblotting. Its stable expression ensures accurate normalization of protein levels, making it a valuable reference protein for ensuring consistency across experimental samples.Beyond its use in WB, beta-tubulin antibodies offered by your website are suitable for immunofluorescence (IF) and immunohistochemistry (IHC) applications. These antibodies enable researchers to visualize microtubule dynamics within different tissues and cell types, providing insight into cellular processes such as migration, division, and intracellular transport. Beta-tubulin's biological relevance extends beyond its role in structural support, as it is essential for processes like spindle formation during mitosis, making it an indispensable marker for studies of cell division and cytoskeletal integrity.
target | type | reactivity | applications | ||||
---|---|---|---|---|---|---|---|
5007 | beta Tubulin | mouse monoclonal (BT7R) | human mouse rat rabbit hamster | WB ICC ELISA | |||
9003 | beta Tubulin | rabbit monoclonal | human mouse rat | WB ICC IHC |
WB with antibody [5007] : 10µg/lane of mouse brain tissue lysates. 50kDa band is Anti-β-Tubulin (5007) at 1:2000 dilution (0.5µg/mL).
WB with antibody [9003] : Detection of mouse beta-Tubulin by Western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
IHC with antibody [9003] : Detection of mouse beta-Tubulin by immunohistochemistry. Sample: FFPE section of mouse testis. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG.