Human BTN2A1&BTN3A1 complex Protein 5023

$375.00 – $1,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail

available size

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SKU: 5023parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q7KYR7(BTN2A1)&O00481(BTN3A1)
express system	HEK293
product tag	C-His, C-Flag
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.
molecular weight	The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human BTN2A1&BTN3A1 complex Protein 5023

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.
Protein names Butyrophilin subfamily 2 member A1
Gene names BTN2A1,BTN2A1 BT2.1 BTF1
Protein family Immunoglobulin superfamily, BTN/MOG family
Mass 59633Da
Subellular location Membrane ; Single-pass type I membrane protein .
Tissues Highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney.
Target Relevance information above includes information from UniProt accession: Q7KYR7
The UniProt Consortium

Data

ELISA with Human BTN2A1&BTN3A1 complex Protein

Immobilized Human BTN3A1&BTN2A1, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.

HPLC of Human BTN2A1&BTN3A1 complex Protein

The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human BTN2A1&BTN3A1 complex Protein

Human BTN2A1&BTN3A1 complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.