ELISA with Cynomolgus CD40/TNFRSF5 Protein 4809

Cynomolgus CD40/TNFRSF5 Protein 4809

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu21-Arg193

SKU: 4809parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	G7PG38
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
molecular weight	The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus CD40/TNFRSF5 Protein 4809

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Protein names Tumor necrosis factor receptor superfamily member 5 (B-cell surface antigen CD40) (CD40L receptor)
Gene names CD40,CD40
Mass 9541Da
Subellular location Membrane ; Single-pass type I membrane protein .
Target Relevance information above includes information from UniProt accession: G7PG38
The UniProt Consortium

Data

ELISA with Cynomolgus CD40/TNFRSF5 Protein

Immobilized Cynomolgus CD40, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 12.6ng/ml determined by ELISA.

The purity of Cynomolgus CD40 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus CD40/TNFRSF5 Protein

Cynomolgus CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.