SPR with Human Transferrin R/CD71 Protein 4779

ELISA with Human Transferrin R/CD71 Protein 4779

Human Transferrin R/CD71 Protein 4779

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Cys89-Phe760

SKU: 4779parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P02786
express system	HEK293
product tag	N-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
molecular weight	The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Transferrin R/CD71 Protein 4779

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
Protein names Transferrin receptor protein 1 (TR) (TfR) (TfR1) (Trfr) (T9) (p90) (CD antigen CD71) [Cleaved into: Transferrin receptor protein 1, serum form (sTfR)]
Protein family Peptidase M28 family, M28B subfamily
Mass 84871Da
Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738). {ECO:0000250, ECO:0000269\|PubMed:26214738, ECO:0000269\|PubMed:26642240, ECO:0000269\|PubMed:3568132}.; (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. {ECO:0000269\|PubMed:17287727, ECO:0000269\|PubMed:18268337}.
Subellular location Cell membrane {ECO:0000269\|PubMed:17081065}; Single-pass type II membrane protein {ECO:0000269\|PubMed:17081065}. Melanosome {ECO:0000269\|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. {ECO:0000269\|PubMed:17081065}.; [Transferrin receptor protein 1, serum form]: Secreted {ECO:0000269\|PubMed:17081065}.
Structure Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3; facilitates TFRC endocytosis in erythroid precursor cells (PubMed:26642240). {ECO:0000269\|PubMed:16325581, ECO:0000269\|PubMed:26642240}.; (Microbial infection) Interacts with Guanarito, Junin and Machupo arenavirus glycoprotein complex (PubMed:17287727, PubMed:18268337). {ECO:0000269\|PubMed:17287727, ECO:0000269\|PubMed:18268337}.
Post-translational modification Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation (PubMed:26214738). Stearoylation does not affect iron uptake (PubMed:26214738). {ECO:0000269\|PubMed:26214738}.; N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. {ECO:0000269\|PubMed:1421756, ECO:0000269\|PubMed:1421757, ECO:0000269\|PubMed:16335952, ECO:0000269\|PubMed:19159218, ECO:0000269\|PubMed:19349973, ECO:0000269\|PubMed:3582362}.; Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).; Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. {ECO:0000269\|PubMed:3582362}.
Target Relevance information above includes information from UniProt accession: P02786
The UniProt Consortium

Data

SPR with Human Transferrin R/CD71 Protein

Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).

ELISA with Human Transferrin R/CD71 Protein

Immobilized Human Transferrin R, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test).

HPLC of Human Transferrin R/CD71 Protein

The purity of Human Transferrin R is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Transferrin R/CD71 Protein

Human Transferrin R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.