Activity assay with Human TFPI Protein 4777

Human TFPI Protein 4777

$285.00 – $950.00

Summary

Expression: HEK293
Active: Yes (catalytic)
Amino Acid Range: Asp29-Lys282

SKU: 4777parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P10646
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Oestrogens influence the pathophysiology and development of hormone-sensitive cancers, such as breast cancer. Tissue factor pathway inhibitor (TFPI) is a serine protease inhibitor of the extrinsic coagulation pathway and has recently been associated with breast cancer cell development.
molecular weight	The protein has a predicted MW of 32.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human TFPI Protein 4777

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Oestrogens influence the pathophysiology and development of hormone-sensitive cancers, such as breast cancer. Tissue factor pathway inhibitor (TFPI) is a serine protease inhibitor of the extrinsic coagulation pathway and has recently been associated with breast cancer cell development.
Protein names Tissue factor pathway inhibitor (TFPI) (Extrinsic pathway inhibitor) (EPI) (Lipoprotein-associated coagulation inhibitor) (LACI)
Gene names TFPI,TFPI LACI TFPI1
Mass 9606Da
Function Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
Subellular location [Isoform Alpha]: Secreted.; [Isoform Beta]: Microsome membrane ; Lipid-anchor, GPI-anchor .
Tissues Mostly in endothelial cells.
Post-translational modification O-glycosylated.
Domain Th
Target Relevance information above includes information from UniProt accession: P10646
The UniProt Consortium

Data

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is < 0.75 nM.

The purity of Human TFPI is greater than 95% as determined by SEC-HPLC.

Human TFPI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.