SPR with Biotinylated Human IL-2 R beta/CD122 Protein 4743

ELISA with Biotinylated Human IL-2 R beta/CD122 Protein 4743

Biotinylated Human IL-2 R beta/CD122 Protein 4743

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala27-Asp239

SKU: 4743parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.
molecular weight	The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human IL-2 R beta/CD122 Protein 4743

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

SPR with Biotinylated Human IL-2 R beta/CD122 Protein

Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.079 µM as determined in SPR assay (Biacore T200).

Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined in SPR assay (Biacore T200).

ELISA with Biotinylated Human IL-2 R beta/CD122 Protein

Immobilized Human IL-15Ra&IL-15, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human IL-2 R beta, His Tag with the EC50 of 0.16µg/ml determined by ELISA (QC Test).

HPLC of Biotinylated Human IL-2 R beta/CD122 Protein

The purity of Biotinylated Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human IL-2 R beta/CD122 Protein

Biotinylated Human IL-2 R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.