SPR with Biotinylated Human KIR3DL3 Protein 4726

Biotinylated Human KIR3DL3 Protein 4726

$525.00 – $1,750.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Glu26-Leu322

SKU: 4726parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.
molecular weight	The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human KIR3DL3 Protein 4726

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.
Protein names Killer cell immunoglobulin-like receptor 3DL3 (CD158 antigen-like family member Z) (Killer cell inhibitory receptor 1) (CD antigen CD158z)
Gene names KIR3DL3,KIR3DL3 CD158Z KIR3DL7 KIRC1
Protein family Immunoglobulin superfamily
Mass 9606Da
Function Receptor on natural killer cells. May inhibit the activity of NK cells thus preventing cell lysis.
Subellular location Cell membrane; Single-pass type I membrane protein.
Target Relevance information above includes information from UniProt accession: Q8N743
The UniProt Consortium

Data

SPR with Biotinylated Human KIR3DL3 Protein

Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human KIR3DL3, His-Avi Tag with an affinity constant of 0.254 µM as determined in SPR assay (Biacore T200).

HPLC of Biotinylated Human KIR3DL3 Protein

The purity of Biotinylated Human KIR3DL3 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human KIR3DL3 Protein

Biotinylated Human KIR3DL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.