ELISA with Human GPC3/Glypican 3 Protein 4448

Human GPC3/Glypican 3 Protein 4448

$240.00 – $800.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln25-His559

SKU: 4448parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P51654
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Glypican-3 is a protein, which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
molecular weight	The protein has a predicted MW of 61.6 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human GPC3/Glypican 3 Protein 4448

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
Protein names Glypican-3 (GTR2-2) (Intestinal protein OCI-5) (MXR7) [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit]
Protein family Glypican family
Mass 65563Da
Function Cell surface proteoglycan (PubMed:14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed:16227623, PubMed:24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed:17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity). {ECO:0000250\|UniProtKB:Q6V9Y8, ECO:0000250\|UniProtKB:Q8CFZ4, ECO:0000269\|PubMed:14610063, ECO:0000269\|PubMed:16227623, ECO:0000269\|PubMed:17549790, ECO:0000269\|PubMed:24496449}.
Subellular location Cell membrane {ECO:0000269\|PubMed:14610063}; Lipid-anchor, GPI-anchor {ECO:0000250\|UniProtKB:P13265}; Extracellular side {ECO:0000250\|UniProtKB:P13265}.
Tissues Detected in placenta (at protein level) (PubMed:32337544). Highly expressed in lung, liver and kidney. {ECO:0000269\|PubMed:32337544, ECO:0000269\|PubMed:8589713}.
Structure Heterodimer; disulfide-linked (PubMed:14610063). Cleavage by a furin-like convertase results in production of alpha and beta chains which form a disulfide-linked heterodimer (PubMed:14610063). Interacts with DPP4 (PubMed:17549790). Interacts with FGF2 (By similarity). Interacts with WNT5A (PubMed:14610063). Also interacts with WNT3A and WNT7B (PubMed:16227623). Interacts with hedgehog protein SHH; the heparan sulfate chains are not required for the interaction (By similarity). Also interacts with hedgehog protein IHH (By similarity). Interacts with CD81 (By similarity). Interacts with Wnt receptors FZD4, FZD7 and FZD8; the heparan sulfate chains are required for the interaction (PubMed:24496449). {ECO:0000250\|UniProtKB:P13265, ECO:0000250\|UniProtKB:Q8CFZ4, ECO:0000269\|PubMed:14610063, ECO:0000269\|PubMed:16227623, ECO:0000269\|PubMed:17549790, ECO:0000269\|PubMed:24496449}.
Post-translational modification O-glycosylated; contains heparan sulfate and/or chondroitin sulfate. {ECO:0000269\|PubMed:14610063, ECO:0000269\|PubMed:32337544}.; Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor (PubMed:14610063). This processing is essential for its role in inhibition of hedgehog signaling (PubMed:25653284). A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface (PubMed:14610063). {ECO:0000269\|PubMed:14610063, ECO:0000269\|PubMed:25653284}.
Target Relevance information above includes information from UniProt accession: P51654
The UniProt Consortium

Data

ELISA with Human GPC3/Glypican 3 Protein

Immobilized Human GPC3, His Tag at 0.5µg/ml (100µl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.

The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human GPC3/Glypican 3 Protein

Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.