Cell based assay with Human LIF Protein 4392

Human LIF Protein 4392

$315.00 – $1,050.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Ser23-Phe202

SKU: 4392parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P15018
express system	HEK293
product tag	No Tag
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
molecular weight	The protein has a predicted MW of 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 0.1EU per μg by the LAL method.

Human LIF Protein 4392

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
Protein names Leukemia inhibitory factor (LIF) (Differentiation-stimulating factor) (D factor) (Melanoma-derived LPL inhibitor) (MLPLI) (Emfilermin)
Gene names LIF,LIF HILDA
Protein family LIF/OSM family
Mass 9606Da
Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Subellular location Secreted.
Target Relevance information above includes information from UniProt accession: P15018
The UniProt Consortium

Data

Immobilized Human LIF at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA.

Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50 for this effect is 1-5 ng/mL.

The purity of Human LIF is greater than 95% as determined by SEC-HPLC.

Human LIF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.