ELISA with SARS-COV-2 Spike S1 (D614G) Protein 4378

SARS-COV-2 Spike S1 (D614G) Protein 4378

$330.00 – $1,100.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln14-Arg683(D614G)

SKU: 4378parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	QHD43416
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
molecular weight	The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

SARS-COV-2 Spike S1 (D614G) Protein 4378

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

ELISA with SARS-COV-2 Spike S1 (D614G) Protein

Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2µg/ml (100µl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.

HPLC of SARS-COV-2 Spike S1 (D614G) Protein

The purity of SARS-COV-2 Spike S1 (D614G) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of SARS-COV-2 Spike S1 (D614G) Protein

SARS-COV-2 Spike S1 (D614G) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.