SPR with Human GFRAL/GFR alpha-like Protein 4320

ELISA with Human GFRAL/GFR alpha-like Protein 4320

Human GFRAL/GFR alpha-like Protein 4320

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ser19-Glu351

SKU: 4320parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q6UXV0
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
molecular weight	The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human GFRAL/GFR alpha-like Protein 4320

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Protein names GDNF family receptor alpha-like
Gene names GFRAL,GFRAL C6orf144 UNQ9356/PRO34128
Protein family GDNFR family
Mass 9606Da
Function Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomitting, and/or loss of appetite in response to various stresses (PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:28953886, PubMed:36630958). The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future (PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:28953886, PubMed:36630958). The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin (PubMed:32661391). Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways (PubMed:31535977, PubMed:32661391). Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (By similarity). The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues (PubMed:32661391).
Subellular location Cell membrane ; Single-pass membrane protein ; Extracellular side .
Tissues Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius (at protein level) (PubMed:28846097, PubMed:28846098). Detected at low levels in testis and adipose tissue (PubMed:28846097).
Structure Interacts (via the extracellular domain) with GDF15 and RET; receptor of GDF15, mediates cellular signaling through interaction with RET after GDF15-binding (PubMed:28846097, PubMed:28846099, PubMed:28953886, PubMed:31535977). Interaction with RET requires previous GDF15-binding (PubMed:28846097, PubMed:28846099).
Post-translational modification Cleaved and inactivated by MMP14, inhibiting the GDF15-GFRAL aversive response.
Target Relevance information above includes information from UniProt accession: Q6UXV0
The UniProt Consortium

Data

SPR with Human GFRAL/GFR alpha-like Protein

Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).

ELISA with Human GFRAL/GFR alpha-like Protein

Immobilized Human GDF15, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).

HPLC of Human GFRAL/GFR alpha like Protein

The purity of Human GFRAL is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human GFRAL/GFR alpha-like Protein

Human GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.