Cynomolgus LILRB4/CD85k/ILT3 Protein 4318

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln22-Glu259

available size

Clear

SKU: 4318parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_015297198
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	LILRB4, also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
molecular weight	The protein has a predicted MW of 27.2 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus LILRB4/CD85k/ILT3 Protein 4318

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
Protein names Leukocyte immunoglobulin-like receptor subfamily B member 4 (B4) (CD85 antigen-like family member K) (Immunoglobulin-like transcript 3) (ILT-3) (Leukocyte immunoglobulin-like receptor 5) (LIR-5) (Monocyte inhibitory receptor HM18) (CD antigen CD85k)
Mass 49356Da
Function Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance (PubMed:11875462). Receptor for FN1 (PubMed:34089617). Receptor for apolipoprotein APOE (PubMed:30333625). Receptor for ALCAM/CD166 (PubMed:29263213). Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:9151699). Inhibits FCGR1A/CD64-mediated monocyte activation by inducing phosphatase-mediated down-regulation of the phosphorylation of multiple proteins including LCK, SYK, LAT and ERK, leading to a reduction in TNF production (PubMed:19833736). This inhibition of monocyte activation occurs at least in part via binding to FN1 (PubMed:34089617). Inhibits T cell proliferation, inducing anergy, suppressing the differentiation of IFNG-producing CD8+ cytoxic T cells and enhancing the generation of CD8+ T suppressor cells (PubMed:16493035, PubMed:19833736, PubMed:29263213). Induces up-regulation of CD86 on dendritic cells (PubMed:19860908). Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation (PubMed:11875462). {ECO:0000269\|PubMed:11875462, ECO:0000269\|PubMed:16493035, ECO:0000269\|PubMed:19833736, ECO:0000269\|PubMed:19860908, ECO:0000269\|PubMed:29263213, ECO:0000269\|PubMed:30333625, ECO:0000269\|PubMed:34089617, ECO:0000269\|PubMed:9151699}.
Subellular location Cell membrane {ECO:0000269\|PubMed:19833736, ECO:0000269\|PubMed:9151699}; Single-pass type I membrane protein {ECO:0000269\|PubMed:9151699}. Note=Ligand binding leads to internalization and translocation to an antigen-processing compartment. {ECO:0000269\|PubMed:9151699}.
Tissues Detected on monocytes, macrophages, dendritic cells, natural killer cells and B-cells (at protein level). Expressed in the lung. {ECO:0000269\|PubMed:19833736, ECO:0000269\|PubMed:30333625, ECO:0000269\|PubMed:9151699, ECO:0000269\|PubMed:9278324, ECO:0000269\|PubMed:9548455}.
Structure Interacts with PTPN6. {ECO:0000269\|PubMed:16493035, ECO:0000269\|PubMed:9151699}.
Domain Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-
Target Relevance information above includes information from UniProt accession: Q8NHJ6
The UniProt Consortium

Data

ELISA with Cynomolgus LILRB4/CD85k/ILT3 Protein

Immobilized Cynomolgus LILRB4, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.

HPLC of Cynomolgus LILRB4/CD85k/ILT3 Protein

The purity of Cynomolgus LILRB4 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus LILRB4/CD85k/ILT3 Protein

Cynomolgus LILRB4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.