ELISA with Human EpCAM/TROP1 Protein 4214

Human EpCAM/TROP1 Protein 4214

$195.00 – $650.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln24-Lys265

SKU: 4214parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P16422
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
molecular weight	The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human EpCAM/TROP1 Protein 4214

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF-like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Protein names Epithelial cell adhesion molecule (Ep-CAM) (Adenocarcinoma-associated antigen) (Cell surface glycoprotein Trop-1) (Epithelial cell surface antigen) (Epithelial glycoprotein) (EGP) (Epithelial glycoprotein 314) (EGP314) (hEGP314) (KS 1/4 antigen) (KSA) (Major gastrointestinal tumor-associated protein GA733-2) (Tumor-associated calcium sign
Gene names EPCAM,EPCAM GA733-2 M1S2 M4S1 MIC18 TACSTD1 TROP1
Protein family EPCAM family
Mass 9606Da
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Subellular location Lateral cell membrane ; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction.
Tissues Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Structure Monomer. Interacts with phosphorylated CLDN7.
Post-translational modification Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Target Relevance information above includes information from UniProt accession: P16422
The UniProt Consortium

Data

Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 µM as determined in SPR assay (Biacore T200).

Immobilized Human EpCAM, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA (QC Test).

The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human EpCAM/TROP1 Protein

Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.