Human IFN gamma R1/IFNGR1 Protein 4204

$150.00 – $500.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu18-Gly245

SKU: 4204parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	AAH05333
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway.Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action.
molecular weight	The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IFN gamma R1/IFNGR1 Protein 4204

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway.Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action.
Protein names Interferon gamma receptor 1 (IFN-gamma receptor 1) (IFN-gamma-R1) (CDw119) (Interferon gamma receptor alpha-chain) (IFN-gamma-R-alpha) (CD antigen CD119)
Protein family Type II cytokine receptor family
Mass 54405Da
Function Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:20015550). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed:10986460, PubMed:2971451, PubMed:7615558, PubMed:7617032, PubMed:7673114). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed:28883123). STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (By similarity). {ECO:0000250\|UniProtKB:P15261, ECO:0000269\|PubMed:10986460, ECO:0000269\|PubMed:20015550, ECO:0000269\|PubMed:28883123, ECO:0000269\|PubMed:2971451, ECO:0000269\|PubMed:7615558, ECO:0000269\|PubMed:7617032, ECO:0000269\|PubMed:7673114}.
Subellular location Cell membrane {ECO:0000269\|PubMed:10811850, ECO:0000269\|PubMed:28883123}; Single-pass type I membrane protein {ECO:0000255}.
Structure Monomer (PubMed:9367779). Heterodimer with IFNGR2, to form the IFNG receptor complex (PubMed:7615558). Interacts with JAK1 (PubMed:7615558). Interacts (when phosphorylated) with STAT1 (PubMed:8156998). Interacts with SOCS1 (By similarity). {ECO:0000250\|UniProtKB:P15261, ECO:0000269\|PubMed:7615558, ECO:0000269\|PubMed:8156998, ECO:0000269\|PubMed:9367779}.
Post-translational modification Phosphorylated at Ser/Thr residues. Phosphorylation of Tyr-457 is required for IFNG receptor signal transduction (PubMed:8156998). Influenza virus infection leads to phosphorylation in a CSNK1A1-dependent manner (PubMed:29343571). {ECO:0000269\|PubMed:29343571, ECO:0000269\|PubMed:7615558, ECO:0000269\|PubMed:7673114, ECO:0000269\|PubMed:8156998}.; Ubiquitinated after phosphorylation in a CSNK1A1-dependent manner, leading to the lysosome-dependent degradation (PubMed:29343571). Proteasomally degraded through 'Lys-48'-mediated ubiquitination (PubMed:28883123). Ubiquitination is necessary for efficient IFNGR1 signaling (PubMed:28883123). {ECO:0000269\|PubMed:28883123, ECO:0000269\|PubMed:29343571}.
Target Relevance information above includes information from UniProt accession: P15260
The UniProt Consortium

ELISA with Human IFN gamma R1/IFNGR1 Protein

Immobilized Human IFN gamma, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.

HPLC of Human IFN gamma R1/IFNGR1 Protein

The purity of Human IFN gamma R1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human IFN gamma R1/IFNGR1 Protein

Human IFNGR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.