Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein 4202

$473.00 – $1,575.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val22-Pro189(Cynomolgus IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)

available size

Clear

SKU: 4202parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A2K5TLV4(Cynomolgus IL3 alpha)&P43432(Mouse IL2 beta)
express system	HEK293
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
molecular weight	The protein has a predicted MW of 19.5 (Cynomolgus IL23 alpha)&35.8 (Mouse IL12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein 4202

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
Protein names Interleukin-23 subunit alpha (Interleukin-23 subunit p19)
Protein family 388
Pathway 282
Subellular location Secreted .
Target Relevance information above includes information from UniProt accession: A0A2K5TLV4
The UniProt Consortium

Data

SPR with Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein

Cynomolgus IL-23R, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 3.90 nM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein

Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test).

HPLC of Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein

The purity of Cynomolgus IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein

Cynomolgus IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.