Human CD74/DHLAG Protein 4187

$240.00 – $800.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln73-Met232

SKU: 4187parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_004346
express system	HEK293
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE
background	CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.
molecular weight	The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD74/DHLAG Protein 4187

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.
Protein names H-2 class II histocompatibility antigen gamma chain (Ia antigen-associated invariant chain) (Ii) (Ii chain) (MHC class II-associated invariant chain) (CD antigen CD74) [Cleaved into: Class-II-associated invariant chain peptide (CLIP)]
Gene names Cd74,Cd74 Ii
Mass 10090Da
Function Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.; [Isoform Long]: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs).; [Class-II-associated invariant chain peptide]: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.
Catalytic activity #N/A
Subellular location [Isoform Long]: Late endosome. Lysosome .; Cell membrane ; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Secreted. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
Tissues [Isoform Long]: Expressed in thymus and lymph noodes (PubMed:8977190). Expressed by antigen-presenting cells (APCs) (PubMed:11483509, PubMed:12417635).; [Isoform Short]: Expressed in thymus and lymph noodes.
Structure Nonamer composed of three alpha/beta/gamma heterotrimers. Interacts with CD44; this complex is essential for the MIF-induced signaling cascade that results in B cell survival.; [Isoform Long]: Interacts with the mature form of CTSL; the complex survive in neutral pH environment.
Target Relevance information above includes information from UniProt accession: P04441
The UniProt Consortium

Data

Immobilized Human CD74 at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

SDS-PAGE gel of Human CD74/DHLAG Protein

Human CD74 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.