Biotinylated Human PD-L2/B7-DC Protein 4096

$525.00 – $1,750.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Leu20-Thr220

SKU: 4096parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
molecular weight	The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human PD-L2/B7-DC Protein 4096

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
Protein names Programmed cell death 1 ligand 2 (PD-1 ligand 2) (PD-L2) (PDCD1 ligand 2) (Programmed death ligand 2) (Butyrophilin B7-DC) (B7-DC) (CD antigen CD273)
Protein family Immunoglobulin superfamily, BTN/MOG family
Mass 30957Da
Function Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity). {ECO:0000250}.
Subellular location [Isoform 3]: Secreted {ECO:0000305\|PubMed:15253154}.; [Isoform 2]: Endomembrane system {ECO:0000269\|PubMed:15253154}; Single-pass type I membrane protein {ECO:0000255}.; [Isoform 1]: Cell membrane {ECO:0000269\|PubMed:15253154}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:Q9WUL5, ECO:0000305\|PubMed:15340161}.
Tissues Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. {ECO:0000269\|PubMed:11224527}.
Structure Interacts with PDCD1. {ECO:0000250}.
Target Relevance information above includes information from UniProt accession: Q9BQ51
The UniProt Consortium

SPR with Biotinylated Human PD-L2/B7-DC Protein

Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 µM as determined in SPR assay (Biacore T200).

HPLC of Biotinylated Human PD-L2/B7-DC Protein

The purity of Biotinylated Human PD-L2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human PD-L2/B7-DC Protein

Biotinylated Human PD-L2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.