Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein 4085

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Met22-Arg734

SKU: 4085parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.
molecular weight	The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein 4085

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.
Protein names Semaphorin-4D (A8) (BB18) (GR3) (CD antigen CD100)
Gene names SEMA4D,SEMA4D C9orf164 CD100 SEMAJ
Protein family Semaphorin family
Mass 9606Da
Function Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed:20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed:19788569). Promotes the migration of cerebellar granule cells (PubMed:16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed:8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed:16055703).
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
Structure Homodimer (PubMed:20877282). Interacts with PLXNB2 (By similarity). Interacts with PLXNB1 (PubMed:10520995).
Target Relevance information above includes information from UniProt accession: Q92854
The UniProt Consortium

ELISA with Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein

Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.

HPLC of Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein

The purity of Biotinylated Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein

Biotinylated Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.