Human CD43 Protein 3981

$300.00 – $1,000.00

Summary

Expression: HEK293
Pure: Yes (SDS-PAGE)
Amino Acid Range: Ser20-Arg253

SKU: 3981parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P16150
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE
background	CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions. The induction of acute myocarditis involves the engagement of CD43 cytoplasmic tripeptide sequence KRR to ezrin-radixin-moiesin cytoskeletal proteins.
molecular weight	The protein has a predicted MW of 24.5 kDa. Due to glycosylation, the protein migrates to 75-120 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD43 Protein 3981

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions. The induction of acute myocarditis involves the engagement of CD43 cytoplasmic tripeptide sequence KRR to ezrin-radixin-moiesin cytoskeletal proteins.
Protein names Leukosialin (GPL115) (Galactoglycoprotein) (GALGP) (Leukocyte sialoglycoprotein) (Sialophorin) (CD antigen CD43) [Cleaved into: CD43 cytoplasmic tail (CD43-ct) (CD43ct)]
Mass 40322Da
Function Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity). {ECO:0000250\|UniProtKB:P15702, ECO:0000269\|PubMed:18036228, ECO:0000269\|PubMed:24328034}.; [CD43 cytoplasmic tail]: Protects cells from apoptotic signals, promoting cell survival. {ECO:0000250\|UniProtKB:P15702}.
Subellular location Membrane {ECO:0000255}; Single-pass type I membrane protein {ECO:0000255}. Cell projection, microvillus {ECO:0000250\|UniProtKB:P13838}. Cell projection, uropodium {ECO:0000250\|UniProtKB:P15702}. Note=Localizes to the uropodium and microvilli via its interaction with ERM proteins (EZR, RDX and MSN). {ECO:0000250\|UniProtKB:P13838, ECO:0000250\|UniProtKB:P15702}.; [CD43 cytoplasmic tail]: Nucleus {ECO:0000269\|PubMed:15003504}. Nucleus, PML body {ECO:0000250\|UniProtKB:P15702}. Note=The sumoylated form localizes to the PML body. {ECO:0000250\|UniProtKB:P15702}.
Tissues Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Structure Interacts with SIGLEC1. {ECO:0000250\|UniProtKB:P15702}.; [CD43 cytoplasmic tail]: Monomer (By similarity). Interacts with CTNNB1 (PubMed:15003504). Interacts with RDX (via FERM domain), EZR and MSN (By similarity). {ECO:0000250\|UniProtKB:P13838, ECO:0000250\|UniProtKB:P15702, ECO:0000269\|PubMed:15003504}.
Post-translational modification Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. {ECO:0000269\|PubMed:1731338}.; Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes. {ECO:0000250\|UniProtKB:P15702}.; Has a high content of sialic acid and O-linked carbohydrate structures.; Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus. {ECO:0000269\|PubMed:15540986, ECO:0000269\|PubMed:18586676}.; [CD43 cytoplasmic tail]: Sumoylated.
Target Relevance information above includes information from UniProt accession: P16150
The UniProt Consortium

Data

Human CD43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.