Human JAML Protein 3947

$300.00 – $1,000.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Leu20-Leu275

SKU: 3947parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_001091996
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	JAML (junctional adhesion molecule-like), contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML mRNA is expressed in hematopoietic tissues and is prominently expressed in granulocytes. The fact that JAML protein is localized at the cell plasma membrane in the areas of cell-cell contacts, whereas it is not detected at free cell borders, suggests that JAML is engaged in homophilic interactions.
molecular weight	The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human JAML Protein 3947

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
JAML (junctional adhesion molecule-like), contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML mRNA is expressed in hematopoietic tissues and is prominently expressed in granulocytes. The fact that JAML protein is localized at the cell plasma membrane in the areas of cell-cell contacts, whereas it is not detected at free cell borders, suggests that JAML is engaged in homophilic interactions.
Protein names Junctional adhesion molecule-like (Adhesion molecule interacting with CXADR antigen 1) (Dendritic cell-specific protein CREA7-1)
Protein family Immunoglobulin superfamily
Mass 44339Da
Function Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR. {ECO:0000269\|PubMed:12869515, ECO:0000269\|PubMed:15800062, ECO:0000269\|PubMed:18948633, ECO:0000269\|PubMed:19064666}.
Subellular location Cell membrane {ECO:0000269\|PubMed:12869515}; Single-pass type I membrane protein {ECO:0000269\|PubMed:12869515}. Cell junction {ECO:0000269\|PubMed:12869515}. Note=Localized at the plasma membrane and enriched in areas of cell-cell contacts (PubMed:12869515).
Tissues Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. Preferentially expressed by mature leukocytes (at protein level). {ECO:0000269\|PubMed:12869515, ECO:0000269\|PubMed:18948633, ECO:0000269\|PubMed:19064666}.
Structure Homodimer; active form in leukocyte-endothelial cell adhesion. Interacts (homodimeric form) with CXADR. Interacts (via cytoplasmic domain) with the PI3 kinase; upon CXADR-binding. Interacts with ITGA4 and ITGB1; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling JAML homodimerization. {ECO:0000269\|PubMed:15800062, ECO:0000269\|PubMed:19064666}.
Domain The Ig-like V-type domain 1 mediates interaction with CXADR (By similarity). The Ig-like V-t
Target Relevance information above includes information from UniProt accession: Q86YT9
The UniProt Consortium

Data

The purity of Human JAML is greater than 95% as determined by SEC-HPLC.

Human JAML on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.